More Subjects
TA Cloning
[Name of the Writer]
[Name of the Institution]
TA Cloning
Abstract
The method of TOPO TA cloning combines the advantages of TA cloning with the ligation activity of topoisomerase 1. In just 5 minutes the direct ligation of PCR products takes place. The key component in TOPO cloning system is the enzyme called topoisomerase 1, this particular enzyme basically works as both restriction enzyme and also as a ligase. The main function of the topoisomerase 1 is to break down and then rejoin the DNA during the replication processes, the penta-sequence is particularly recognized by the vaccinia virus topoisomerase 1 and then it forms a covalent bond with the phosphate group which is then attached to the 3ʹ thymidine . By unwinding the DNA strand it enables the DNA to open up. Once the DA strands are opened then the enzymes relegate the ends of the opened DNA strands and then it releases itself from the DNA. In order to bind the relegating activity of the topoisomerase certain vectors are provided that linearized with the topoisomerase 1 covalently bound with the 3ʹ phosphate group of the DNA. As a result, the vectors are enabled to ligate the DNA sequences at the well-matched ends. The entire process of ligation is completed within 5 minutes at a specific room temperature. The PCR product is produced by following the PCR setup, that contains the DNA template of the sample, PCR Buffer, dNTP mix, primers and sterile water. This is a competent method of producing a cell within the given controlled conditions of the laboratory.
Introduction
Many of the New York graveyards have been flattened for various development over the years. The burial domes at the Spring Street Presbyterian Church were four concealed rooms during the 200 years which passed since the domes were used, the coffins that were made up of woods were stacked within them and they were decayed and collapsed due to which the masses of bones and other objects were exposed during the entire process of diggings. The domes were completely forgotten but the unique history of this church again came into the limelight due to the rediscovery of the vault. The remains that were recovered from this site were the only large collection of human remains that dates back to the 19th century. After identification of the remains, it became clear that approximately 75 of the dead bodies were children and their death occurred due to diseases like rickets and upon morphological identification, it became clear that all the individuals belong to diverse backgrounds and they were buried together in the domes. The DNA samples that were extracted from these bones or bodies helped the scientists to identify some of the main facts about these corpses including how they died and also their ancestors .
References
ADDIN ZOTERO_BIBL {"uncited":[],"omitted":[],"custom":[]} CSL_BIBLIOGRAPHY “Cloning - Cloning Methods - TA and TOPO TA Cloning - EMBL.” Accessed April 19, 2019. https://www.embl.de/pepcore/pepcore_services/cloning/cloning_methods/topo_ta/.
French, Mary. “Spring Street Presbyterian Church Burial Vaults.” New York City Cemetery Project (blog), August 26, 2018. https://nycemetery.wordpress.com/2018/08/26/spring-street-presbyterian-church-burial-vaults/.
More Subjects
Join our mailing list
© All Rights Reserved 2024